Review



lambda dna bsteii digest new  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    New England Biolabs lambda dna bsteii digest new
    Construction of M2 mutant viruses and corresponding marker rescue viruses. (A) The MHV68 genome cloned as a BAC (2) was used to generate all M2 recombinant viruses by RecA-mediated recombination. For M2 mutant viruses exhibiting robust establishment-of-latency and/or reactivation-from-latency phenotypes, a marker rescue virus in which the wt M2 sequences were targeted to the M2 ORF by allelic exchange to restore the wt phenotype was generated. The <t>DNA</t> probe used for the Southern blot analyses contained sequence from the M2 ORF (genomic coordinates, bp 4031 to 4627). Relevant restriction sites are indicated. A, AluI; Bg, BglI; D, DdeI; N, NcoI; BU, BstUI; Bs, BssHII. (B and C) Southern blot analyses of recombinant viruses harboring mutations in the indicated PXXP motifs of M2, along with corresponding marker rescue viruses. (D) Southern blot analyses of recombinant viruses harboring mutations in the indicated tyrosine residues of M2 and their corresponding marker rescue viruses. (E) Southern blot analysis of M2.Stop(2). 32P-labeled molecular size standards <t>(Lambda</t> <t>DNA-BstEII</t> digest; New England Biolabs, Beverly, MA) were included in each Southern blot analysis. WT, wt MHV68; MR, marker rescue virus.
    Lambda Dna Bsteii Digest New, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lambda dna bsteii digest new/product/New England Biolabs
    Average 94 stars, based on 24 article reviews
    lambda dna bsteii digest new - by Bioz Stars, 2026-03
    94/100 stars

    Images

    1) Product Images from "Systematic Mutagenesis of the Murine Gammaherpesvirus 68 M2 Protein Identifies Domains Important for Chronic Infection ▿ "

    Article Title: Systematic Mutagenesis of the Murine Gammaherpesvirus 68 M2 Protein Identifies Domains Important for Chronic Infection ▿

    Journal:

    doi: 10.1128/JVI.02234-07

    Construction of M2 mutant viruses and corresponding marker rescue viruses. (A) The MHV68 genome cloned as a BAC (2) was used to generate all M2 recombinant viruses by RecA-mediated recombination. For M2 mutant viruses exhibiting robust establishment-of-latency and/or reactivation-from-latency phenotypes, a marker rescue virus in which the wt M2 sequences were targeted to the M2 ORF by allelic exchange to restore the wt phenotype was generated. The DNA probe used for the Southern blot analyses contained sequence from the M2 ORF (genomic coordinates, bp 4031 to 4627). Relevant restriction sites are indicated. A, AluI; Bg, BglI; D, DdeI; N, NcoI; BU, BstUI; Bs, BssHII. (B and C) Southern blot analyses of recombinant viruses harboring mutations in the indicated PXXP motifs of M2, along with corresponding marker rescue viruses. (D) Southern blot analyses of recombinant viruses harboring mutations in the indicated tyrosine residues of M2 and their corresponding marker rescue viruses. (E) Southern blot analysis of M2.Stop(2). 32P-labeled molecular size standards (Lambda DNA-BstEII digest; New England Biolabs, Beverly, MA) were included in each Southern blot analysis. WT, wt MHV68; MR, marker rescue virus.
    Figure Legend Snippet: Construction of M2 mutant viruses and corresponding marker rescue viruses. (A) The MHV68 genome cloned as a BAC (2) was used to generate all M2 recombinant viruses by RecA-mediated recombination. For M2 mutant viruses exhibiting robust establishment-of-latency and/or reactivation-from-latency phenotypes, a marker rescue virus in which the wt M2 sequences were targeted to the M2 ORF by allelic exchange to restore the wt phenotype was generated. The DNA probe used for the Southern blot analyses contained sequence from the M2 ORF (genomic coordinates, bp 4031 to 4627). Relevant restriction sites are indicated. A, AluI; Bg, BglI; D, DdeI; N, NcoI; BU, BstUI; Bs, BssHII. (B and C) Southern blot analyses of recombinant viruses harboring mutations in the indicated PXXP motifs of M2, along with corresponding marker rescue viruses. (D) Southern blot analyses of recombinant viruses harboring mutations in the indicated tyrosine residues of M2 and their corresponding marker rescue viruses. (E) Southern blot analysis of M2.Stop(2). 32P-labeled molecular size standards (Lambda DNA-BstEII digest; New England Biolabs, Beverly, MA) were included in each Southern blot analysis. WT, wt MHV68; MR, marker rescue virus.

    Techniques Used: Mutagenesis, Marker, Clone Assay, Recombinant, Generated, Southern Blot, Sequencing, Labeling, Lambda DNA Preparation



    Similar Products

    lambda dna bsteii digest new  (New England Biolabs)
    94
    New England Biolabs lambda dna bsteii digest new
    Construction of M2 mutant viruses and corresponding marker rescue viruses. (A) The MHV68 genome cloned as a BAC (2) was used to generate all M2 recombinant viruses by RecA-mediated recombination. For M2 mutant viruses exhibiting robust establishment-of-latency and/or reactivation-from-latency phenotypes, a marker rescue virus in which the wt M2 sequences were targeted to the M2 ORF by allelic exchange to restore the wt phenotype was generated. The <t>DNA</t> probe used for the Southern blot analyses contained sequence from the M2 ORF (genomic coordinates, bp 4031 to 4627). Relevant restriction sites are indicated. A, AluI; Bg, BglI; D, DdeI; N, NcoI; BU, BstUI; Bs, BssHII. (B and C) Southern blot analyses of recombinant viruses harboring mutations in the indicated PXXP motifs of M2, along with corresponding marker rescue viruses. (D) Southern blot analyses of recombinant viruses harboring mutations in the indicated tyrosine residues of M2 and their corresponding marker rescue viruses. (E) Southern blot analysis of M2.Stop(2). 32P-labeled molecular size standards <t>(Lambda</t> <t>DNA-BstEII</t> digest; New England Biolabs, Beverly, MA) were included in each Southern blot analysis. WT, wt MHV68; MR, marker rescue virus.
    Lambda Dna Bsteii Digest New, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lambda dna bsteii digest new/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    lambda dna bsteii digest new - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Construction of M2 mutant viruses and corresponding marker rescue viruses. (A) The MHV68 genome cloned as a BAC (2) was used to generate all M2 recombinant viruses by RecA-mediated recombination. For M2 mutant viruses exhibiting robust establishment-of-latency and/or reactivation-from-latency phenotypes, a marker rescue virus in which the wt M2 sequences were targeted to the M2 ORF by allelic exchange to restore the wt phenotype was generated. The DNA probe used for the Southern blot analyses contained sequence from the M2 ORF (genomic coordinates, bp 4031 to 4627). Relevant restriction sites are indicated. A, AluI; Bg, BglI; D, DdeI; N, NcoI; BU, BstUI; Bs, BssHII. (B and C) Southern blot analyses of recombinant viruses harboring mutations in the indicated PXXP motifs of M2, along with corresponding marker rescue viruses. (D) Southern blot analyses of recombinant viruses harboring mutations in the indicated tyrosine residues of M2 and their corresponding marker rescue viruses. (E) Southern blot analysis of M2.Stop(2). 32P-labeled molecular size standards (Lambda DNA-BstEII digest; New England Biolabs, Beverly, MA) were included in each Southern blot analysis. WT, wt MHV68; MR, marker rescue virus.

    Journal:

    Article Title: Systematic Mutagenesis of the Murine Gammaherpesvirus 68 M2 Protein Identifies Domains Important for Chronic Infection ▿

    doi: 10.1128/JVI.02234-07

    Figure Lengend Snippet: Construction of M2 mutant viruses and corresponding marker rescue viruses. (A) The MHV68 genome cloned as a BAC (2) was used to generate all M2 recombinant viruses by RecA-mediated recombination. For M2 mutant viruses exhibiting robust establishment-of-latency and/or reactivation-from-latency phenotypes, a marker rescue virus in which the wt M2 sequences were targeted to the M2 ORF by allelic exchange to restore the wt phenotype was generated. The DNA probe used for the Southern blot analyses contained sequence from the M2 ORF (genomic coordinates, bp 4031 to 4627). Relevant restriction sites are indicated. A, AluI; Bg, BglI; D, DdeI; N, NcoI; BU, BstUI; Bs, BssHII. (B and C) Southern blot analyses of recombinant viruses harboring mutations in the indicated PXXP motifs of M2, along with corresponding marker rescue viruses. (D) Southern blot analyses of recombinant viruses harboring mutations in the indicated tyrosine residues of M2 and their corresponding marker rescue viruses. (E) Southern blot analysis of M2.Stop(2). 32P-labeled molecular size standards (Lambda DNA-BstEII digest; New England Biolabs, Beverly, MA) were included in each Southern blot analysis. WT, wt MHV68; MR, marker rescue virus.

    Article Snippet: 32 P-labeled molecular size standards (Lambda DNA-BstEII digest; New England Biolabs, Beverly, MA) were included in each Southern blot analysis.

    Techniques: Mutagenesis, Marker, Clone Assay, Recombinant, Generated, Southern Blot, Sequencing, Labeling, Lambda DNA Preparation